Recipe

Ni-NTA Wash Buffer

Reagent Quantity (for 500 mL) Final concentration
Ni-NTA lysis buffer (5×)       100 mL    1×
β-Mercaptoethanol (14.1 m) 177.5 µL, add fresh  5 mm
Imidazole (1 m, pH 8.0)         5 mL 10 mm
ddH2O      to 500 mL
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec078907 Cold Spring Harb Protoc 2013: pdb.rec078907- © 2013 Cold Spring Harbor Laboratory Press
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue