High-Yield Transcription Buffer (10×)
| Reagent | Quantity (for 1 mL) |
Final concentration (10×) |
|---|---|---|
| HEPES–KOH (2 m, pH 7.5) | 0.5 mL | 1 m |
| MgCl2 (1 m) | 0.1 mL | 0.1 m |
| Spermidine–HCl (1 m) | 20 µL | 0.02 m |
| DTT (2 m) | 0.2 mL | 0.4 m |
| Bovine serum albumin (BSA), RNase-free (20 mg/mL) | 50 µL | 1 mg/mL |
| H2O | 130 µL | |
| Store for up to 1 wk at −20°C. | ||
- © 2013 Cold Spring Harbor Laboratory Press
