Recipe

Drosophila Adult Hemolymph-Like Saline (AHLS)

Reagent Final concentration (mm)
CaCl2   2
KCl   5
HEPES   5
MgCl2  8.2
NaCl 108
NaHCO3   4
NaH2PO4   1
Sucrose   10
Trehalose   5
Adjust the pH to 7.5. Filter. Store at 4°C. Measure the osmolarity of this solution using a freezing-point osmometer. The osmolarity should be 265 mOsm, very close to the value for adult Drosophila hemolymph (Singleton and Woodruff 1994). Use solutions of CaCl2 and MgCl2 instead of powders to avoid precipitation in the saline.

REFERENCES

    1. Singleton K,
    2. Woodruff RI
    Singleton K, Woodruff RI. 1994. The osmolarity of adult Drosophila hemolymph and its effect on oocyte nurse cell electrical polarity. Dev Biol 161: 154–167.
    CrossRefMedlineGoogle Scholar
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  1. doi:10.1101/pdb.rec079459 Cold Spring Harb Protoc 2013: pdb.rec079459- © 2013 Cold Spring Harbor Laboratory Press
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