Reverse Transcription Buffer (5×)
| Reagent | Quantity (for 1 mL) (µL) |
Final concentration (mm) |
|---|---|---|
| MgCl2 (1 m) | 30 | 30 |
| DTT (1 m) | 100 | 100 |
| dNTPs (25 mm each) | 100 | 2.5 |
| H2O | 770 | |
| Store for up to 6 mo at −20°C. | ||
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