Modification Reaction Buffer A (Denaturing)
| Reagent | Quantity (for 100 mL) (mL) |
Final concentration (mm) |
|---|---|---|
| HEPES/KOH (1 m, pH 7.5) | 1 | 10 |
| EDTA (0.5 m, pH 8) | 0.5 | 1 |
| H2O | 98.5 | |
| Store indefinitely at room temperature. | ||
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