Modification Reaction Buffer B (Denaturing)
| Reagent | Quantity (for 100 mL) (mL) |
Final concentration (mm) |
|---|---|---|
| Potassium borate (0.2 m, pH 7.9) | 5 | 10 |
| EDTA (0.2 m, pH 8.0) | 0.5 | 1 |
| H2O | 94.5 | |
| Store indefinitely at 4°C. | ||
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