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Infrared Video Microscopy for Visualizing Neurons and Neuronal Excitation in Brain Slices

  1. Walter Zieglgänsberger
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

Here we describe methods for imaging neurons and neuronal excitation in brain slices with infrared video microscopy. Patch clamping in conjunction with a gradient-contrast system allows electrical recording from fine neuronal processes. Imaging with an infrared (IR)-darkfield system allows visualization of the intrinsic optical signal (IOS).

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  1. doi:10.1101/pdb.prot079434 Cold Spring Harb Protoc 2013: pdb.prot079434- © 2013 Cold Spring Harbor Laboratory Press
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