Recipe

Annealing Buffer (2×)

Reagent Quantity (µL) (for 1 mL) Final concentration (mm)
KCl (2 m) 250 500
Tris (1 m, pH 8.3)  50  50
H2O 700
Store indefinitely at −20°C.
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  1. doi:10.1101/pdb.rec080069 Cold Spring Harb Protoc 2013: pdb.rec080069- © 2013 Cold Spring Harbor Laboratory Press
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