Formamide Gel-Loading Buffer
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Deionized formamide | 9.5 mL | 95% |
| Bromophenol blue | 2.5 mg | 0.025% (w/v) |
| Xylene cyanol FF | 2.5 mg | 0.025% (w/v) |
| EDTA (0.5 m, pH 8.0) | 100 µL | 5 mm |
| H2O | 400 µL | |
| Store in aliquots at –20°C. | ||
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