Amphibian Ringer’s Solution (200–250 mOsm)

doi:10.1101/pdb.rec073684

Recipe

Amphibian Ringer’s Solution (200–250 mOsm)

6.6 g NaCl

0.15 g KCl

0.15 g CaCl₂

0.2 g NaHCO₃

Add the reagents to 1 L of distilled water. The solution may be stored for up to 7 d, or longer if it is autoclaved and stored in sterile containers.

An alternative formulation consists of 6.5 g NaCl, 0.42 g KCl, and 0.25 g CaCl₂ added to 1 L of distilled water. Up to 10 g of glucose may be added to either formulation, but this reduces the shelf life of unsterilized solution to 24 h.

Source: Wright (2006).

REFERENCE

↵
1. Wright K
Wright K. 2006. Important clinical aspects of amphibian physiology. Proceedings of the North American Veterinary Conference Vol. 20. Orlando, Florida.
Google Scholar