External Saline
| Reagent | Final concentration |
|---|---|
| NaCl | 103 mm |
| KCl | 3 mm |
| CaCl2 | 1.5 mm |
| MgCl2 | 4 mm |
| NaH2PO4 | 1 mm |
| NaHCO3 | 26 mm |
| TES (N-tris[hydroxymethyl]methyl-2- aminoethane sulfonic acid) |
5 mm |
| Trehalose | 10 mm |
| Glucose | 10 mm |
| Sucrose | 9 mm |
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1. Adjust the osmolarity of the solution to 275–280 mOsm by changing the concentration of sucrose, which is not metabolized by the brain and acts as an inert solute here.
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2. Oxygenate by bubbling 95% O2/5% CO2 continuously through the solution. The pH should equilibrate to 7.3.
- © 2013 Cold Spring Harbor Laboratory Press
