Recipe

Reverse Transcription (RT) Buffer

100 µg/mL BSA (bovine serum albumen)

0.5 mm dCTP, dGTP, dATP, dTTP

10 mm DTT (dithiothreitol)

25 mm KCl

3.5 mm MgCl2

50 mm Tris–HCl (7.5)

Store at −20°C.

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  1. doi:10.1101/pdb.rec073767 Cold Spring Harb Protoc 2013: pdb.rec073767- © 2013 Cold Spring Harbor Laboratory Press
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