Recipe

T4 PNK Buffer (10×)

50 mm DTT (dithiothreitol)

1 mm EDTA (pH 8.0)

0.1 m MgCl2

1 mm spermidine HCl

0.5 m Tris-HCl (pH 7.6)

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  1. doi:10.1101/pdb.rec073791 Cold Spring Harb Protoc 2013: pdb.rec073791- © 2013 Cold Spring Harbor Laboratory Press
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