Recipe

Klenow Buffer (5×)

Reagent Quantity (for 1 mL) Final concentration
NaCl (5 m) 50 µL 250 mm
Tris–HCl (1 m, pH 7.9) 50 µL 50 mm
DTT (dithiothreitol; 1 m) 5 µL 5 mm
H2O 895 µL
Store for 6 mo at −20°C.
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  1. doi:10.1101/pdb.rec073809 Cold Spring Harb Protoc 2013: pdb.rec073809- © 2013 Cold Spring Harbor Laboratory Press

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