Klenow Buffer (5×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| NaCl (5 m) | 50 µL | 250 mm |
| Tris–HCl (1 m, pH 7.9) | 50 µL | 50 mm |
| DTT (dithiothreitol; 1 m) | 5 µL | 5 mm |
| H2O | 895 µL | |
| Store for 6 mo at −20°C. | ||
- © 2013 Cold Spring Harbor Laboratory Press
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| NaCl (5 m) | 50 µL | 250 mm |
| Tris–HCl (1 m, pH 7.9) | 50 µL | 50 mm |
| DTT (dithiothreitol; 1 m) | 5 µL | 5 mm |
| H2O | 895 µL | |
| Store for 6 mo at −20°C. | ||