NT2 Buffer
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 7.4) | 5 mL | 50 mm |
| NaCl (5 m) | 3 mL | 150 mm |
| MgCl2 (1 m) | 0.1 mL | 1 mm |
| Nonidet P-40 (NP-40; 10%) | 0.5 mL | 0.05% |
| H2O | 91.4 mL | |
| Store for 6 mo at 4°C. | ||
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