Reverse Transcriptase Buffer for SELEX (10×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.3) | 500 µL | 500 mm |
| KCl (2 m) | 200 µL | 400 mm |
| MgCl2 (1 m) | 60 µL | 60 mm |
| DTT (dithiothreitol; 1 m) | 10 µL | 10 mm |
| H2O | 230 µL | |
| Store for 6 mo at –20°C. | ||
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