Topic Introduction

An Optical Fiber-Based Uncaging System

  1. Richard S. Givens
Adapted from Imaging in Neuroscience (ed. Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

This article describes the assembly and performance of a simple and inexpensive ultraviolet-flash system suitable for rapid focal photolysis of caged compounds in cultured neurons and brain slices. Advantages and limitations of this system are discussed. Examples are provided illustrating how this system can be used for stimulating neurons and mapping their functional inputs in brain slices.

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  1. doi:10.1101/pdb.top072900 Cold Spring Harb Protoc 2013: pdb.top072900- © 2013 Cold Spring Harbor Laboratory Press
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