SDS–PAGE Protein Sample Buffer (2×)
| Reagent | Quantity (for 50 mL) |
Final concentration |
|---|---|---|
| Tris (1 m, pH 6.8) | 4.0 mL | 80 mm |
| SDS (20%) | 5 mL | 2.0% |
| Glycerol | 5 mL | 10% |
| Bromophenol blue (0.1%) | 300 μL | 0.0006% |
| DTT (1 m) | 5 mL | 0.1 m |
| H2O | 30.7 mL | |
| Omit DTT for longer-term storage. Store indefinitely at room temperature. Add DTT to 0.1 m in aliquots for daily use. |
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