Recipe

Holtfreter Buffer

NaCl 3.5 g
CaCl2 0.1 g
KCl 0.05 g
HEPES (1 m, pH 7.5) 1 mL
Adjust to a final volume of 1 L. Store at room temperature.
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  1. doi:10.1101/pdb.rec074005 Cold Spring Harb Protoc 2013: pdb.rec074005- © 2013 Cold Spring Harbor Laboratory Press
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