Oocyte Dissociation Solution
| Reagent | Final concentration |
|---|---|
| NaCl | 88 mm |
| KCl | 2.5 mm |
| Na2HPO4 | 10 mm |
| HEPES | 5 mm |
| Supplement with collagenase (∼1–2 mg/mL; Type 1A, Sigma-Aldrich) when ready to use. Oocyte viability may be better preserved with lower collagenase concentrations and longer incubation periods at lower temperature. |
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