Recipe

Embryo Time-Lapse Solution

HEPES (0.1 m, pH 7.3) 1 mL
MS-222 (tricaine methane sulfonate; 0.4%) 1 mL
Phenylthiourea (0.12%) 250 μL
Embryo medium (E3M; 1×) to 10 mL
Make fresh on the day of use and keep at room temperature or prewarm at 30°C.
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  1. doi:10.1101/pdb.rec074039 Cold Spring Harb Protoc 2013: pdb.rec074039- © 2013 Cold Spring Harbor Laboratory Press
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