Phosphatase Buffer (10×)
| Reagent | Quantity (for 1 mL) |
Final concentration (10×) |
|---|---|---|
| Tris–HCl (1 m, pH 7.9) | 0.5 mL | 0.5 m |
| MgCl2 (1 m) | 0.1 mL | 100 mm |
| NaCl (5 m) | 0.2 mL | 1 m |
| DTT (1 m) | 10 μL | 10 mm |
| H2O | 0.19 mL | |
| Store for up to 6 mo at −20°C. | ||
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