Rapid Ligation Mix (2×)
| Reagent | Quantity (for 1 mL) |
Final concentration (2×) |
|---|---|---|
| Tris–HCl (1 m, pH 7.5) | 132 μL | 132 mm |
| MgCl2 (1 m) | 20 μL | 20 mm |
| DTT (1 m) | 2 μL | 2 mm |
| ATP (100 mm) | 20 μL | 2 mm |
| Polyethylene glycol (PEG) (50%, w/v) | 300 μL | 15% (w/v) |
| H2O | 526 μL | |
| Store for up to 6 mo at –20°C. Note that rapid ligation buffers are available from many commercial suppliers. |
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