TBST for Western Blotting
| Reagent | Final concentration (1×) |
|---|---|
| NaCl | 137 mm |
| KCl | 2.7 mm |
| Tris base | 19 mm |
| Prepare a 20× stock of TBS by combining appropriate amounts of the ingredients listed above. Autoclave and store at room temperature. Prepare a working solution of TBS (1×, pH 7.4) by diluting the 20× stock in Milli-Q water. For TBST, add 1 mL of Tween 20 per liter of TBS. Stir constantly until homogenous. |
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