Reaction Buffer A (Denaturing)
| Reagent | Quantity (for 100 mL) |
Final concentration |
|---|---|---|
| HEPES/KOH (1 m, pH 7.5) | 1 mL | 10 mm |
| EDTA (0.2 m, pH 8) | 0.5 mL | 1 mm |
| H2O | 98.5 mL | |
| Store indefinitely at room temperature. | ||
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