Recipe

CNTF Stock (10 μg/mL)

To prepare, dilute ciliary neurotrophic factor (CNTF; Peprotech 450-13) to 10 μg/mL with sterile 0.2% BSA in Dulbecco's phosphate-buffered saline (D-PBS; HyClone SH30264.01). Make 20-μL aliquots, flash freeze in liquid nitrogen, and store at –80°C.

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  1. doi:10.1101/pdb.rec074724 Cold Spring Harb Protoc 2013: pdb.rec074724- © 2013 Cold Spring Harbor Laboratory Press
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