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Tips for Preparing mRNA-Seq Libraries from Poly(A)+ mRNA for Illumina Transcriptome High-Throughput Sequencing

  1. Brenton R. Graveley
Adapted from RNA: A Laboratory Manual, by Donald C. Rio, Manuel Ares Jr, Gregory J. Hannon, and Timothy W. Nilsen. CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

Many investigators who do high-throughput sequencing of mRNA (mRNA-Seq) use kits for library preparation purchased from Illumina. Recognizing that these kits are continually being updated and improved, we provide here some background information, tips, and troubleshooting advice for the kits available at the time of this writing.

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  1. doi:10.1101/pdb.ip073908 Cold Spring Harb Protoc 2013: pdb.ip073908- © 2013 Cold Spring Harbor Laboratory Press
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