Microinjecting Holo-Aequorin into Dechorionated and Intact Zebrafish Embryos
- 1Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China;
- 2Marine Biological Laboratory, Woods Hole, Massachusetts 02543
Abstract
The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca2+ to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.
Footnotes
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↵3 Correspondence: barnie{at}ust.hk
- © 2013 Cold Spring Harbor Laboratory Press
