Recipe

Internal Pipette Solution

Reagent Final concentration
K-gluconate 140 mm
NaCl  4 mm
Mg-ATP  4 mm
Na-GTP  0.3 mm
HEPES buffer   10 mm
Adjust the pH to 7.2–7.4 with KOH.
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  1. doi:10.1101/pdb.rec075119 Cold Spring Harb Protoc 2013: pdb.rec075119- © 2013 Cold Spring Harbor Laboratory Press
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