Recipe

Stop Buffer for DNase I Footprinting

400 mm sodium acetate

0.2% SDS

10 mm EDTA

50 µg/mL yeast tRNA

The stop buffer terminates the DNase I reaction and prepares it for phenol extraction and ethanol precipitation. EDTA is added to chelate divalent cations necessary for DNase I activity, SDS denatures proteins and strips them from DNA, and sodium acetate and carrier tRNA facilitate ethanol precipitation. This solution can be prepared and maintained as a concentrated stock for several months at room temperature.

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  1. doi:10.1101/pdb.rec075242 Cold Spring Harb Protoc 2013: pdb.rec075242- © 2013 Cold Spring Harbor Laboratory Press
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