Stop Buffer for DNase I Footprinting
400 mm sodium acetate
0.2% SDS
10 mm EDTA
50 µg/mL yeast tRNA
The stop buffer terminates the DNase I reaction and prepares it for phenol extraction and ethanol precipitation. EDTA is added to chelate divalent cations necessary for DNase I activity, SDS denatures proteins and strips them from DNA, and sodium acetate and carrier tRNA facilitate ethanol precipitation. This solution can be prepared and maintained as a concentrated stock for several months at room temperature.
- © 2013 Cold Spring Harbor Laboratory Press
