Recipe

RNase III Buffer (10×)

Reagent Quantity (for 10 mL) Final concentration (10×)
NaCl (5 m)  1 mL 500 mm
Tris–Cl (1 m, pH 7.9)  1 mL 100 mm
MgCl2 (1 m)  1 mL 100 mm
DTT (1 m) 0.1 mL  10 mm
dH2O 6.9 mL
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  1. doi:10.1101/pdb.rec075333 Cold Spring Harb Protoc 2013: pdb.rec075333- © 2013 Cold Spring Harbor Laboratory Press
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