Low Ovomucoid Stock Solution (10×)

To prepare, add 3 g of BSA (Sigma-Aldrich A8806) to 150 mL D-PBS. Mix well. Add 3 g of trypsin inhibitor (Worthington LS003086) and mix to dissolve. Add ∼1 mL of 1 n NaOH to adjust the pH to 7.4. Bring the volume to 200 mL with D-PBS. Filter-sterilize through a 0.22-μm filter. Make 1.0-mL aliquots and store at −20°C.

• © 2013 Cold Spring Harbor Laboratory Press