High Ovomucoid Stock Solution (10×)

To prepare, add 6 g of BSA (Sigma-Aldrich A8806) to 150 mL D-PBS. Add 6 g of trypsin inhibitor (Worthington LS003086) and mix to dissolve. Add at least 1.5 mL of 1 n NaOH to adjust the pH; continue adding NaOH as necessary to bring up the pH to 7.4. Bring the volume to 200 mL with D-PBS. Filter-sterilize through a 0.22-μm filter. Make 1.0-mL aliquots and store at −20°C.

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