Inhibitor Stock Solution
| Reagent | Volume | Final concentration |
|---|---|---|
| EBSS (10×) (Sigma-Aldrich E7510) | 50 mL | 1× |
| d(+)-glucose (30%) | 6 mL | 0.46% |
| NaHCO3 (1 m) | 13 mL | 26 mm |
| ddH2O | 170.4 mL | |
| Bring the volume to 500 mL with ddH2O and filter-sterilize through a 0.22-μm filter. Store at 4°C. | ||
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