Recipe

Inhibitor Stock Solution

Reagent Volume Final concentration
EBSS (10×) (Sigma-Aldrich E7510)   50 mL    1×
d(+)-glucose (30%)   6 mL   0.46%
NaHCO3 (1 m)  13 mL   26 mm
ddH2O 170.4 mL
Bring the volume to 500 mL with ddH2O and filter-sterilize through a 0.22-μm filter. Store at 4°C.
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  1. doi:10.1101/pdb.rec075648 Cold Spring Harb Protoc 2013: pdb.rec075648- © 2013 Cold Spring Harbor Laboratory Press
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