Enzyme Stock Solution
| Reagent | Volume | Final concentration |
|---|---|---|
| EBSS (10×) (Sigma-Aldrich E7510) | 20 mL | 1× |
| d(+)-Glucose (30%) | 2.4 mL | 0.46% |
| NaHCO3 (1 m) | 5.2 mL | 26 mm |
| EDTA (50 mm) | 2 mL | 0.5 mm |
| ddH2O | 170.4 mL | |
| Bring the volume to 200 mL with ddH2O and filter-sterilize through a 0.22-μm filter. Store at 4°C. | ||
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