Recipe

Buffer B (for Microinjection)

Reagent Final concentration
NaCl 140 mm
KCl  5 mm
MgSO4  1 mm
CaCl2  1 mm
NaH2PO4  1 mm
Glucose 5.5 mm
HEPES  20 mm
Adjust pH to 7.4.
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  1. doi:10.1101/pdb.rec074690 Cold Spring Harb Protoc 2013: pdb.rec074690- © 2013 Cold Spring Harbor Laboratory Press

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