Buffer A (Hypotonic)
| Reagent | Quantity (for 500 mL) | Final concentration |
|---|---|---|
| Tris (1 m, pH 7.9) | 5 mL | 10 mm |
| MgCl2 (1 m) | 0.75 mL | 1.5 mm |
| KCl (3 m) | 1.6 mL | 10 mm |
| H2O | to 500 mL | |
| Add 0.5 mm DTT to 10–20 mL. Store the remainder for 1 yr at 4°C. | ||
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