Buffer D (Dialysis)
| Reagent | Quantity (for 2 L) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 7.9) | 40 mL | 20 mm |
| Glycerol (100%, v/v) | 400 mL | 20% (v/v) |
| KCl (3 m) | 66.6 mL | 100 mm |
| EDTA (0.5 m, pH 8.0) | 0.8 mL | 0.2 mm |
| Dithiothreitol (DTT; 1 M) | 2 mL | 1 mm |
| H2O | to 2 L | |
| Carefully pour the glycerol into the bottom of a 2-L measuring cylinder so that it does not run down the sides. To store buffers for later use, make them without DTT and add fresh DTT to the portions used. Prepare buffers the day before so that they will be well chilled. | ||
- © 2013 Cold Spring Harbor Laboratory Press
