High-Ovomucoid Stock (6×)

• 1. Dissolve the following in 160–180 mL of Dulbecco’s phosphate-buffered saline (D-PBS; Invitrogen 14287-080).

  Reagent	Amount (for 200 mL)	Final concentration
  BSA (Sigma-Aldrich A-8806)	6 g	30 mg/mL
  Trypsin inhibitor  (Worthington LS003086)	6 g	30 mg/mL

• 2. Adjust the pH to 7.4 with 10 n NaOH. Bring the volume to 200 mL with D-PBS, and then filter-sterilize.

• 3. Make 1-mL aliquots and store them at −20°C.

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