BDNF Stock (50 µg/mL)

Recipe

1. Prepare a master BDNF stock (1 mg/mL) by resuspending 1 mg of human brain-derived neurotrophic factor in powder form (BDNF; Peprotech 450-02) in 1 mL of cold, sterile 0.2% BSA (Sigma-Aldrich A-4161) that was prepared in D-PBS (Gibco 14287). Make 200-µL aliquots of the master stock, flash-freeze in liquid nitrogen, and store at −80°C.
2. To make a working BDNF stock, thaw a 200-µL aliquot of master stock on ice. At the same time, chill 3.8 mL of sterile 0.2% BSA solution on ice. Once chilled, add the master BDNF stock to the 0.2% BSA solution and mix well, but gently, to avoid foaming. Make 20-µL working aliquots and flash-freeze in liquid nitrogen. Store at −80°C.