Recipe

RGC Growth Medium

DMEM-Sato base growth medium (with NB) 20 mL
Forskolin stock (4.2 mg/mL) 20 µL
BDNF stock (50 µg/mL) 20 µL
Ciliary neurotrophic factor (10 µg/mL) 20 µL
Warm growth medium to 37°C prior to cell culture, or store at 4°C for up to 3 d.
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  1. doi:10.1101/pdb.rec075986 Cold Spring Harb Protoc 2013: pdb.rec075986- © 2013 Cold Spring Harbor Laboratory Press
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