T4 DNA Ligase Buffer (10×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.5) | 0.5 mL | 500 mm |
| MgCl2 (1 m) | 0.1 mL | 100 mm |
| DTT (1 m) | 0.1 mL | 100 mm |
| ATP (100 mm) | 0.1 mL | 10 mm |
| H2O | 0.2 mL | |
| Store for 6 mo at −20°C. | ||
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