Recipe

Buffer D+

20 mm HEPES-KOH (pH 7.9)

20% (v/v) glycerol

0.2 mm EDTA

0.1 m KCl

Just before use, add 1 mm DTT and 0.5 mm phenylmethylsulfonylfluoride (PMSF).

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  1. doi:10.1101/pdb.rec076737 Cold Spring Harb Protoc 2013: pdb.rec076737- © 2013 Cold Spring Harbor Laboratory Press
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