Table 3.

Summary of sampling scheme for estimating synapse number and size

AP used to estimate VsrCA1 2500 mm2
T used to estimate VsrCA1 300 µm
Sections used to estimate VsrCA1 12
EM stacks per mouse 6
s sections per EM stack 8–14
Aframe, EM 3.3 × 108 mm2
t, EM 100 nm
Graphic for estimate of NSYN 210 (146–295)
Graphic for estimate of ASYN 181 (126–247)
  • (VsrCA1) Volume of stratum radiatum CA1 of hippocampus, (AP) area per point on point grid, (T) distance between corresponding surfaces of slabs used to estimate VsrCA1, (s) sections in a stack, (Aframe) area of disector counting frame, (t) section thickness, (Graphic) sum of synapses counted in six stacks.

This Article

  1. Cold Spring Harb Protoc 2013: pdb.top071886-