Cover image

Series of confocal time-lapse images of Arabidopsis root tip cells expressing the Ca²⁺ probe Yellow Cameleon 3.6 (YC3.6) in the cytosol. The Ca²⁺ signal is propagated as a wave from the external cells to the internal cells after ATP treatment. The cytoplasmic calcium concentration ([Ca²⁺]_cyt) rise is measured as FRET change and is presented in false color (green to red). In this issue, Jörg Kudla and colleagues describe how to analyze Ca²⁺ dynamics in vivo in plants using Yellow Cameleon Ca²⁺ indicators (doi: 10.1101/pdb.top066183). Image courtesy of Alex Costa.