Live Cell Imaging of Cytoplasmic and Nuclear Ca2+ Dynamics in Arabidopsis Roots
- Department of Developmental Biology, Centre for Organismal Studies (COS), University of Heidelberg, 69120 Heidelberg, Germany
Abstract
Approaches for Ca2+ measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM-based experiment to measure cytosolic and nuclear Ca2+ dynamics in Arabidopsis lines expressing Yellow Cameleon Ca2+ indicators.
Footnotes
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↵1 Correspondence: melanie.krebs{at}cos.uni-heidelberg.de
- © 2013 Cold Spring Harbor Laboratory Press
