NT-3 Stock (1 µg/mL)

Recipe

1. Prepare a master stock of neurotrophin-3 (NT-3; Peprotech 450-03) at 1–0.1 mg/mL according to manufacturer’s instructions. (Instructions may vary from lot to lot; generally, NT-3 is dissolved in buffer [e.g., Dulbecco’s phosphate-buffered saline] plus 0.2% BSA.) Store at −80°C.
2. Prepare 0.2% BSA (Sigma-Aldrich A4161) in Dulbecco’s phosphate-buffered saline (D-PBS; Invitrogen 14287-080). Filter-sterilize and chill.
3. Dilute an aliquot of NT-3 master stock to 1 µg/mL in sterile, chilled 0.2% BSA prepared in Step 2.
4. Aliquot the NT-3 working stock from Step 3 (e.g., 20 µL/tube) and snap-freeze in liquid nitrogen.
5. Store aliquots at −80°C.