T3 Stock (4 µg/mL)
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1. Dissolve 4 mg of thyroid hormone (T3; Sigma-Aldrich T6397) in 500 µL of 1 n NaOH to prepare a solution of 0.8 mg/100 µL.
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2. Add 75 µL of the T3 solution from Step 1 to 150 mL of Dulbecco’s phosphate-buffered saline (D-PBS; Invitrogen 14287-080).
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3. Filter solution through a filter-sterilization unit, discarding the first 10 mL.
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4. Aliquot (e.g., 200 µL/tube) and then store at −20°C.
